Description
Overview
| Product name: | 2-AG(2-Arachidonoylglycerol) ELISA Kit |
| Reactivity: | General |
| Alternative Names: | 2-AG |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 1.41 ng/mL |
| Standard: | 300 ng/mL |
| Detection Range: | 4.69-300 ng/mL |
| Sample Type: | Serum, plasma and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Neuro science |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 2-AG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 2-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 2-AG in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard curve
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Manual
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