Support: +1 (203) 804-1318

PCR Cloning & Subcloning

Amplify a target sequence and clone or subclone it into the vector you specify, with the construct Sanger-verified before shipment. Use it to move a gene into a new vector, capture a sequence from a template, or build a construct at a defined insertion site. Custom primer design supports cloning into any site.

Key Features

  • Cloning and subcloning into any vector at the site you specify
  • Custom primer design for difficult or directional inserts
  • Full insert Sanger-verified before shipment

We guarantee 100% accurate cloning. If the delivered construct doesn’t match your design, we will remake it at no additional cost.

Pricing & Turnaround

Gene Length Turnaround Starting Price
<1000 bp 10–12 business days $119
1000–2000 bp 12–14 business days $149
2001–3000 bp 14–16 business days $199
>3000 bp Upon request Quote

Plasmids over 3 kb are quoted individually.

Sample Requirements

  1. Validated DNA sequence (validation fees apply if Genzera will do the validation)
  2. Commercial or custom vector with complete sequence information
  3. Specialized reagents, customer-provided or purchased by Genzera with extra charge

Deliverables

  • 2–5 μg lyophilized plasmid DNA
  • Sequencing chromatogram (.ab1)
  • Certificate of Analysis

Get Started Today

Request a quote with your sequence and project details, and a scientist will review it and follow up with pricing and turnaround.

Have additional questions or a difficult cloning project? Call +1 (203) 804-1318 or email [email protected]. Our team of PhD researchers with decades of experience can help.