Support: +1 (203) 804-1318

Lentivirus Packaging

Lentiviral packaging for stable gene delivery, from plasmid construction through titered, ready-to-use virus. The VSV-G-pseudotyped lentiviral vector, derived from HIV-1 with its virulence genes replaced, integrates your gene into the host genome for long-term expression and infects both dividing and non-dividing cells. We package overexpression, RNAi, and CRISPR sgRNA constructs at medium and large scale.

Target cells: neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, and stem cells, including primary cells.

Illustration of the lentivirus packaging process.

Key Features

  • Broad tropism: infects dividing and non-dividing cells, including primary cells
  • Stable expression: genomic integration maintains expression through cell division
  • Replication-defective: the recombinant strain cannot replicate autonomously
  • Resistant to silencing: integrated transgenes resist gene-silencing mechanisms
  • Payload: carries inserts up to ~4.8 kb

Every batch is covered by our 100% quality guarantee. If the virus doesn’t meet the titer and specification you ordered, we will remake it at no additional cost.

Pricing & Turnaround

ScalePackageTurnaroundPrice
MediumOver-expression (≤3 kb), ≥2×10⁸ TU/ml, 200 μl20–25 business daysQuote
MediumRNAi, single target, ≥2×10⁸ TU/ml, 200 μl20–25 business daysQuote
MediumRNAi, 3 targets (1 guaranteed), ≥2×10⁸ TU/ml, 200 μl20–25 business daysQuote
MediumsgRNA, single target, ≥2×10⁸ TU/ml, 200 μl20–25 business daysQuote
MediumsgRNA, 3 targets, ≥2×10⁸ TU/ml, 200 μl20–25 business daysQuote
LargeOver-expression (≤3 kb), ≥10⁹ TU/ml, 200 μl25–30 business daysQuote
LargeRNAi, ≥10⁹ TU/ml, 200 μl25–30 business daysQuote
LargesgRNA, single target, ≥10⁹ TU/ml, 200 μl25–30 business daysQuote

Turnaround includes plasmid construction and preparation.

Deliverables

Custom lentivirus, to specification:

  • Medium scale: ≥10⁸ TU/ml, 5×200 μl
  • Large scale: ≥10⁹ TU/ml, 5×200 μl

Included at no charge:

  • Control virus (medium >10⁸ TU/ml; large >10⁹ TU/ml, 5×200 μl)
  • Polybrene, 10 mg/ml, 1×200 μl

Storage: dry ice / −80°C; avoid repeated freeze-thaw.

Get Started Today

Request a quote with your construct and project details, and a scientist will review it and follow up with pricing and turnaround.

Have additional questions or a custom packaging job? Call +1 (203) 804-1318 or email [email protected]. Our team of PhD researchers with decades of experience can help.