Advanced functional genomics screening support through specialized pooled screening quality control, deconvolution, and analysis services for CRISPR, shRNA, and related pooled perturbation workflows.
Genzera collaborates with experienced gene modulation and functional genomics partners to provide expertise in library representation, guide/barcode quantification, enrichment and depletion analysis, bottleneck assessment, and workflow interpretation. Services are optimized specifically for pooled screening applications.
Every project is covered by our 100% quality guarantee. If a run doesn’t meet the agreed QC specifications, we will repeat it at no additional cost.
Workflow
- Plasmid Pool QC by NGS — Optional early evaluation of library representation, construct distribution, and pool complexity before screening
- Conduct Your Screen — Perform pooled screening with preferred cell lines and libraries (CRISPR knockout, activation/interference, shRNA, or related systems)
- Ship Frozen Cell Pellets — Send screened samples to Genzera
- Genomic DNA Extraction & NGS Library Preparation — High-quality DNA extraction and optimized sequencing library preparation
- Next-Generation Sequencing — High-throughput NGS with flexible depth and configurations
- Deconvolution & Bioinformatics Analysis — Read alignment, guide/barcode quantification, enrichment/depletion analysis, and comparative analysis
Key Advantages
- Specialized expertise in pooled functional genomics
- Optimized workflows for cost efficiency and reliability
- Screening-focused analysis beyond standard NGS
- Pre-screen plasmid pool QC available
- Experienced partner collaboration
Deliverables
- Raw NGS sequencing data
- Processed guide/barcode count tables
- Enrichment and depletion comparative analysis
- Quality control and library representation metrics
- Construct abundance and pool complexity assessment
- Ready-to-analyze datasets
Applications
- CRISPR knockout screens
- CRISPR activation (CRISPRa) screens
- CRISPR interference (CRISPRi) screens
- shRNA knockdown screens
- Drug resistance studies
- Functional genomics research
- Target discovery programs
- Cancer biology screening studies
- Pooled perturbation and barcode-based workflows
Turnaround
~4 weeks for standard projects, depending on scale, sequencing requirements, and analysis complexity. Expedited timelines available upon request.
Get Started Today
Request a quote with your screen and project details, and a scientist will review it and follow up with pricing and turnaround.
Have additional questions or a complex screen? Call +1 (203) 804-1318 or email [email protected]. Our team of PhD researchers with decades of experience can help.