Description
Overview
| Product name: | 12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit |
| Reactivity: | General |
| Alternative Names: | 12HETE |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 5.13 ng/mL |
| Standard: | 1000 ng/mL |
| Detection Range: | 15.63-1000 ng/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard curve
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Manual
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