Description

LB Broth, Lennox is a highly referenced, nutrient-rich microbial growth medium optimized for molecular biology applications. Featuring a low salt formulation, this dry powder medium is ideal for the cultivation of salt-sensitive E. coli strains used in cloning, plasmid production, and recombinant protein expression.

Derived from the original Lennox formulation, it contains half the sodium chloride concentration of LB Miller, offering a more suitable environment for select vectors and salt-sensitive conditions.

Composition

Component	Amount (g/L)
Tryptone	10.0
Yeast Extract	5.0
Sodium Chloride	5.0
Final pH (25°C)	7.0 ± 0.2

 

Storage Conditions

• Store at 10–30°C
• Shelf life: 3 years (unopened)

Applications

• Non-selective growth of E. coli strains for cloning and DNA preparation
• Selective growth when combined with low-salt compatible antibiotics (e.g., Zeocin®)
• General molecular biology and microbiology workflows
• Academic teaching labs and research use

Instructions for Use

1. Weigh 25g of powder
2. Dissolve in 1L of distilled or deionized water
3. Stir and heat to boiling until fully dissolved
4. Dispense into flasks or tubes
5. Autoclave at 121°C for 20 minutes

Features

• Low-salt formulation for sensitive strains
• Widely cited in molecular biology protocols
• Powder format allows easy weighing and scaling
• Cost-effective alternative to liquid media
• Packed in convenient 250g wide-mouth bottles

Comparison: Lennox vs. Miller

Feature	LB Lennox	LB Miller
NaCl content	5 g/L	10 g/L
Osmolarity	Lower	Higher
Use Case	Salt-sensitive applications	Standard E. coli culture
Growth Behavior	Mild	Robust

 

Cross Reference

• BD: 240230
• Sigma-Aldrich: L3022
• Teknova: L9195

Ordering Information

Catalog #: SD7005

Description: LB Broth (Lennox), powder medium

Packaging: 250g/bottle, 10 bottles/case

For bulk orders or technical inquiries, contact us at [email protected]