Description
PhaseShield™ Gel Tubes are centrifuge tubes pre-loaded with a density-matched phase lock gel for phenol/chloroform and TRIzol nucleic acid extraction. During centrifugation the gel forms a stable barrier above the organic phase, trapping the interphase and contaminants below it. The aqueous phase then decants or pipettes off in a single step, with up to 30% higher recovery and no organic reagent contamination.
This format handles larger sample volumes, such as tissue homogenates and whole blood.
Key Advantages
- Up to 30% higher recovery: recover the full aqueous phase in one pour
- No measurable carryover: organic-reagent-free fraction for downstream reactions
- Stable barrier on every spin: consistent gel layer across sample types and conditions
- Inert, heat-stable polymer: does not inhibit reverse transcriptase, polymerases, or ligases
- Reduced solvent exposure: one pour-off instead of repeated pipetting
- Drop-in compatibility: works with any phenol/chloroform or TRIzol® workflow, with minimal protocol changes
- 100% quality guarantee: if a batch doesn’t perform, we replace it free
Density Selection
Heavy — plasmid DNA, RNA, and viscous, high-impurity samples. Not for pure (saturated) phenol extraction.
Light — restriction enzyme digestion, cDNA synthesis, labeling reactions, and conventional tissue genomic DNA (except rat tail).
| Aqueous Phase | Phenol/CHCl₃ | CHCl₃ | Water/Buffers | Sat. Phenol |
|---|---|---|---|---|
| <0.5 M NaCl | H, L | H, L | H, L | L |
| <1 mg/ml BSA | H, L | H, L | H, L | L |
| Plasmid DNA | H | H | H | — |
| Tissue homogenate DNA | H, L | H, L | H, L | L |
| RNA extraction | H | H | H | L |
Centrifugation Conditions (15 mL)
| Rotor Type | G-Force | Time |
|---|---|---|
| Fixed-angle | 1,500–2,000 × g | 3–5 min |
| Swinging-bucket | 1,500–2,500 × g | 3–5 min |
First use: centrifuge gel tubes briefly to seat the gel at the bottom before adding sample.
Applications
Heavy
- Recovery of DNA from agarose gel electrophoresis.
- M13/Phagemid DNA Separation and Purification.
- Isolation and purification of Lambda DNA.
- Genomic DNA extraction from whole blood.
- Extraction of DNA from cells in tissue culture.
- Extraction of genomic DNA from the mouse tail.
- Extraction of plasmid DNA by traditional method.
- Extraction of total RNA from various samples.
Light
- Recovery of DNA from agarose gel electrophoresis.
- Isolation and purification of Lambda DNA.
- Genomic DNA extraction from whole blood.
- Extraction of DNA from cells in tissue culture.
- Extraction of genomic DNA from the mouse tail.
Specifications
- Component: 50 × 15 mL tubes
- Densities: Heavy or Light
- Storage: Room temperature
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