Background

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.

Images

	Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). 14-3-3 alpha/beta expression in wild-type (WT) and YWHAB knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody (Cat#71174, 1:2,000). Green, isotype control; red, 14-3-3 alpha/beta.
	Immunocytochemical staining of HepG2 cells with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.