Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	tripartite motif containing 7
Gene Symbol:	TRIM7
Synonyms:	GNIP; RNF90
Gene ID:	81786
UniProt ID:	Q9C029
Clone ID:	25GB690
Immunogen:	Recombinant protein of human TRIM7
Dilution:	WB 1:400-1:2,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1, a B-box type 2, and a coiled-coil region. The protein localizes to both the nucleus and the cytoplasm, and may represent a participant in the initiation of glycogen synthesis. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-TRIM7 antibody (Cat#71166). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM7 antibody (Cat#71166, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-TRIM7 antibody (Cat#71166). TRIM7 expression in wild type (WT) and TRIM7 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM7 antibody (Cat#71166, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of TRIM7 expression in HeLa cells using anti-TRIM7 antibody (Cat#71166, 1:2,000). Green, isotype control; red, TRIM7.