Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	calcium/calmodulin dependent protein kinase I
Gene Symbol:	CAMK1
Synonyms:	CAMKI
Gene ID:	8536
UniProt ID:	Q14012
Clone ID:	25GB570
Immunogen:	A synthesized peptide derived from human CaMKI
Dilution:	WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.

Images

	Western blotting analysis using anti-CAMK1 antibody (Cat#71153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAMK1 antibody (Cat#71153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-CAMK1 antibody (Cat#71153). CAMK1 expression in wild-type (WT) and CAMK1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CAMK1 antibody (Cat#71153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of CAMK1 expression in HeLa cells using anti-CAMK1 antibody (Cat# 71153, 1:2,000). Green, isotype control; red, CAMK1.