Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	Brain derived neurotrophic factor
Gene Symbol:	BDNF
Synonyms:	ANON2; BULN2
Gene ID:	627
UniProt ID:	P23560
Clone ID:	23GB3005
Immunogen:	A synthesized peptide derived from human BDNF
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The gene BDNF encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer’s, Parkinson’s, and Huntington’s disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.

Images

	Western blotting analysis using anti-Brain derived neurotrophic factor antibody (Cat#69354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Brain derived neurotrophic factor antibody (Cat#69354, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Brain derived neurotrophic factor antibody (Cat#69354). Brain derived neurotrophic factor expression in wild type (WT) and Brain derived neurotrophic factor shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Brain derived neurotrophic factor antibody (Cat#69354, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Brain derived neurotrophic factor expression in HepG2 cells using Brain derived neurotrophic factor antibody (Cat#69354, 1:2,000). Green, isotype control; red, Brain derived neurotrophic factor.
	Immunocytochemical staining of HepG2 cells with Brain derived neurotrophic factor antibody (Cat#69354, 1:1,000). Nuclei were stained blue with DAPI; Brain derived neurotrophic factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.