Description

Reactivity:	Mouse
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	integrin subunit beta 3
Gene Symbol:	ITGB3
Synonyms:	GT; GT2; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; BDPLT24
Gene ID:	3690
UniProt ID:	P05106
Clone ID:	23GB900
Immunogen:	A synthesized peptide derived from human Integrin beta 3
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Images

	Western blotting analysis using anti-Integrin β3 antibody (Cat#68733). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Integrin β3 antibody (Cat#68733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-integrin beta 3 antibody (Cat#68733). Integrin beta 3 expression in wild-type (WT) and integrin beta 3 (ITGB3) shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-integrin beta 3 antibody (Cat#68733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Integrin beta 3 expression in C2C12 cells using Integrin beta 3 antibody (Cat#68733, 1:2,000). Green, isotype control; red, Integrin beta 3.
	Immunocytochemical staining of C2C12 cells with anti-Integrin beta 3 antibody (Cat#68733, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.