Background

The protein encoded by the gene CASP3 is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer’s disease.

Images

	Western blotting analysis using anti-pro Caspase 3 antibody (Cat#68673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pro Caspase 3 antibody (Cat#68673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-pro Caspase 3 antibody (Cat#68673). pro Caspase 3 expression in wild type (WT) and pro Caspase 3 shRNA knockdown (KD) HAP1 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-pro Caspase 3 antibody (Cat#68673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of pro Caspase 3 in HAP-1 cells using pro Caspase 3 antibody (Cat#68673, 1:2,000). Green, isotype control; red, pro Caspase 3.
	Immunocytochemical staining of HAP-1 cells with pro Caspase 3 antibody (Cat#68673, 1:1,000). Nuclei were stained blue with DAPI; pro Caspase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.