Description

Reactivity:	Human
Applications:	WB
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	transferrin receptor
Gene Symbol:	TFRC
Synonyms:	T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
Gene ID:	7037
UniProt ID:	P02786
Clone ID:	25GB4540
Immunogen:	Recombinant protein of human TFRC
Dilution:	WB 1:1,000-1:5,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.

Images

Western blotting analysis using anti-transferrin receptor antibody (Cat#65617). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-transferrin receptor antibody (Cat#65617, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-transferrin receptor antibody (Cat#65617). Transferrin receptor expression in wild-type (WT) and transferrin receptor (TFRC) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-transferrin receptor antibody (Cat#65617, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

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