Description

Reactivity:	Human
Applications:	WB
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	SET and MYND domain containing 4
Gene Symbol:	SMYD4
Synonyms:	ZMYND21
Gene ID:	114826
UniProt ID:	Q8IYR2
Clone ID:	25GB4235
Immunogen:	Recombinant protein of human SMYD4
Dilution:	WB 1:400-1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Predicted to enable histone deacetylase binding activity. Involved in heart development. Predicted to be active in cytoplasm and nucleus.

Images

Western blotting analysis using anti-SMYD4 antibody (Cat#65555). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMYD4 antibody (Cat#65555, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-SMYD4 antibody (Cat#65555). SMYD4 expression in wild-type (WT) and SMYD4 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMYD4 antibody (Cat#65555, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

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