Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	signal sequence receptor subunit 1
Gene Symbol:	SSR1
Synonyms:	TRAPA
Gene ID:	6745
UniProt ID:	P43307
Clone ID:	25GB1955
Immunogen:	A synthesized peptide derived from human TRAP alpha
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-SSR1 antibody (Cat#65535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SSR1 antibody (Cat#65535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-SSR1 antibody (Cat#65535). SSR1 expression in wild-type (WT) and SSR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SSR1 antibody (Cat#65535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of SSR1 expression in HepG2 cells using anti-SSR1 antibody (Cat#65535, 1:2,000). Green, isotype control; red, SSR1.