Description

Reactivity:	Human, Rat
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG
Clonality:	Oligoclonal antibody
Gene Name:	adducin 1
Gene Symbol:	ADD1
Synonyms:	ADDA
Gene ID:	118
UniProt ID:	P35611
Immunogen:	Recombinant protein of human ADD1
Dilution:	WB 1:500-1:2,500; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C.

Images

	Western blotting analysis using anti-adducin 1 antibody (Cat#65299). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adducin 1 antibody (Cat#65299, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-adducin 1 antibody (Cat#65299). Adducin 1 expression in wild-type (WT) and adducin 1 (ADD1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adducin 1 antibody (Cat#65299, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Adducin 1 expression in HT-1080 cells using anti-Adducin 1 antibody (Cat#65299, 1:2,000). Green, isotype control; red, Adducin 1.