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KD-Validated HEXIM1 Mouse mAb (20 μl)

$149.00

Cat. #: 65265

Description

Reactivity: Human, Mouse, Rat
Applications: WB, FC
Host Species: Mouse
Isotype: IgG2a
Clonality: Monoclonal antibody
Gene Name: HEXIM P-TEFb complex subunit 1
Gene Symbol: HEXIM1
Synonyms: CLP1; EDG1; HIS1; MAQ1
Gene ID: 10614
UniProt ID: O94992
Clone ID: 25GB650
Immunogen: Recombinant protein of human HEXIM1
Dilution: WB 1:400-1:2,000; FC 1:2,000
Purification Method: Affinity purified
Concentration: Lot dependent
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.


Background

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.


Images

HEXIM1 antibody Western blotting analysis using anti-HEXIM1 antibody (Cat#65265). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HEXIM1 antibody (Cat#65265, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
65265 WB KD 1 Western blotting analysis using anti-HEXIM1 antibody (Cat#65265). HEXIM1 expression in wild-type (WT) and HEXIM1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HEXIM1 antibody (Cat#65265, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
65265 FCM 1 Flow cytometric analysis of HEXIM1 expression in HepG2 cells using anti-HEXIM1 antibody (Cat#65265, 1:2,000). Green, isotype control; red, HEXIM1.


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