Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	phosphoinositide-3-kinase regulatory subunit 4
Gene Symbol:	PIK3R4
Synonyms:	p150; VPS15
Gene ID:	30849
UniProt ID:	Q99570
Clone ID:	25GB325
Immunogen:	A synthesized peptide derived from human PIK3R4
Dilution:	WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Predicted to enable protein serine/threonine kinase activity. Involved in several processes, including early endosome to late endosome transport; phosphatidylinositol 3-kinase/protein kinase B signal transduction; and phosphatidylinositol-3-phosphate biosynthetic process. Located in late endosome and microtubule cytoskeleton. Part of phosphatidylinositol 3-kinase complex, class III.

Images

	Western blotting analysis using anti-PIK3R4 antibody (Cat#65244). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3R4 antibody (Cat#65244, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-PIK3R4 antibody (Cat#65244). PIK3R4 expression in wild-type (WT) and PIK3R4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3R4 antibody (Cat#65244, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of PIK3R4 expression in HepG2 cells using anti-PIK3R4 antibody (Cat#65244, 1:2,000). Green, isotype control; red, PIK3R4.