Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	RAP1B, member of RAS oncogene family
Gene Symbol:	RAP1B
Synonyms:	K-REV; RAL1B; THC11
Gene ID:	5908
UniProt ID:	P61224
Clone ID:	25GB210
Immunogen:	A synthesized peptide derived from human RAP1A/B
Dilution:	WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9.

Images

	Western blotting analysis using anti-RAP1B antibody (Cat#65217). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-RAP1B antibody (Cat#65217). RAP1B expression in wild-type (WT) and RAP1B shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of RAP1B expression in HepG2 cells using anti-RAP1B antibody (Cat#65217, 1:2,000). Green, isotype control; red, RAP1B.