Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	poly(A) binding protein interacting protein 1
Gene Symbol:	PAIP1
Synonyms:	Polyadenylate-binding protein-interacting protein 1; PABP-interacting protein 1; PAIP-1; Poly(A)-binding protein-interacting protein 1
Gene ID:	10605
UniProt ID:	Q9H074
Clone ID:	25GB195
Immunogen:	A synthesized peptide derived from human PAIP1
Dilution:	WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene interacts with poly(A)-binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.

Images

	Western blotting analysis using anti-PAIP1 antibody (Cat#65214). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-PAIP1 antibody (Cat#65214). PAIP1 expression in wild-type (WT) and PAIP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of PAIP1 expression in HepG2 cells using anti-PAIP1 antibody (Cat#65214, 1:2,000). Green, isotype control; red, PAIP1.