Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	casein kinase 1 alpha 1
Gene Symbol:	CSNK1A1
Synonyms:	CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
Gene ID:	1452
UniProt ID:	P48729
Clone ID:	25GB125
Immunogen:	A synthesized peptide derived from human Casein Kinase 1 alpha
Dilution:	WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Enables protein serine/threonine kinase activity. Involved in several processes, including peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; and regulation of signal transduction. Located in cytoskeleton; cytosol; and nucleus. Part of beta-catenin destruction complex. Biomarker of Alzheimer’s disease and inclusion body myositis.

Images

	Western blotting analysis using anti-CSNK1A1 antibody (Cat#65200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CSNK1A1 antibody (Cat#65200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-CSNK1A1 antibody (Cat#65200). CSNK1A1 expression in wild-type (WT) and CSNK1A1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CSNK1A1 antibody (Cat#65200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of CSNK1A1 expression in C2C12 cells using anti-CSNK1A1 antibody (Cat#65200, 1:2,000). Green, isotype control; red, CSNK1A1.