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KD-Validated UBE2Z Mouse mAb (20 μl)

$149.00

Cat. #: 65095

Description

Reactivity: Human, Mouse, Rat
Applications: WB, FC
Host Species: Mouse
Isotype: IgG2b
Clonality: Monoclonal antibody
Gene Name: ubiquitin conjugating enzyme E2 Z
Gene Symbol: UBE2Z
Synonyms: USE1; HOYS7
Gene ID: 65264
UniProt ID: Q9H832
Clone ID: 24GB14290
Immunogen: Recombinant protein of human UBE2Z
Dilution: WB 1:400-1:2,000
FC 1:1,000
Purification Method: Affinity purified
Concentration: Lot dependent
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.


Background

This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis.


Images

UBE2Z antibody Western blotting analysis using anti-UBE2Z antibody (Cat#65095). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2Z antibody (Cat#65095, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
65095 WB KD 1 Western blotting analysis using anti-UBE2Z antibody (Cat#65095). UBE2Z expression in wild-type (WT) and UBE2Z shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UBE2Z antibody (Cat#65095, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
65095 FCM 1 Flow cytometric analysis of UBE2Z expression in HepG2 cells using anti-UBE2Z antibody (Cat#65095, 1:1,000). Green, isotype control; red, UBE2Z.


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