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KD-Validated ASRGL1 Mouse mAb (20 μl)

$149.00

Cat. #: 65088

Description

Reactivity: Human
Applications: WB, FC
Host Species: Mouse
Isotype: IgG2a
Clonality: Monoclonal antibody
Gene Name: asparaginase and isoaspartyl peptidase 1
Gene Symbol: ASRGL1
Synonyms: ALP; ALP1; CRASH
Gene ID: 80150
UniProt ID: Q7L266
Clone ID: 24GB14255
Immunogen: Recombinant protein of human ASRGL1
Dilution: WB 1:400-1:2,000; FC 1:2,000
Purification Method: Affinity purified
Concentration: Lot dependent
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.


Background

Enables asparaginase activity and beta-aspartyl-peptidase activity. Involved in asparagine catabolic process via L-aspartate. Located in cytoplasm.


Images

ASRGL1 antibody Western blotting analysis using anti-ASRGL1 antibody (Cat#65088). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ASRGL1 antibody (Cat#65088, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
65088 WB KD 1 Western blotting analysis using anti-ASRGL1 antibody (Cat#65088). ASRGL1 expression in wild-type (WT) and ASRGL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ASRGL1 antibody (Cat#65088, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
65088 FCM 1 Flow cytometric analysis of ASRGL1 expression in HeLa cells using anti-ASRGL1 antibody (Cat#65088, 1:2,000). Green, isotype control; red, ASRGL1.


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