Description
| Reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | N-acetylglucosamine kinase |
| Gene Symbol: | NAGK |
| Synonyms: | GNK; HSA242910 |
| Gene ID: | 55577 |
| UniProt ID: | Q9UJ70 |
| Clone ID: | 24GB14215 |
| Immunogen: | Recombinant protein of human NAGK |
| Dilution: | WB 1:400-1:2,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars.
Images
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Western blotting analysis using anti-N-acetylglucosamine kinase antibody (Cat#65080). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-N-acetylglucosamine kinase antibody (Cat#65080, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-N-acetylglucosamine kinase antibody (Cat#65080). N-acetylglucosamine kinase expression in wild-type (WT) and N-acetylglucosamine kinase (NAGK) knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-N-acetylglucosamine kinase antibody (Cat#65080, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of N-acetylglucosamine kinase expression in HepG2 cells using anti-N-acetylglucosamine kinase antibody (Cat#65080, 1:2,000). Green, isotype control; red, N-acetylglucosamine kinase. |
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