Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	interferon gamma receptor 1
Gene Symbol:	IFNGR1
Synonyms:	CD119; IFNGR; IMD27A; IMD27B
Gene ID:	3459
UniProt ID:	P15260
Clone ID:	24GB13660
Immunogen:	A synthesized peptide derived from human IFNGR1
Dilution:	WB 1:1,000-1:5,000; FC 1:1,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.

Images

	Western blotting analysis using anti-interferon gamma receptor 1 antibody (Cat#64900). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-interferon gamma receptor 1 antibody (Cat#64900). Interferon gamma receptor 1 expression in wild-type (WT) and interferon gamma receptor 1 (IFNGR1) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Interferon gamma receptor 1 expression in C2C12 cells using anti-Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Green, isotype control; red, Interferon gamma receptor 1.
	Immunocytochemical staining of C2C12 cells with anti-Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Nuclei were stained blue with DAPI; Interferon gamma receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.