Description

Reactivity:	Human
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	glutathione S-transferase kappa 1
Gene Symbol:	GSTK1
Synonyms:	GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13
Gene ID:	373156
UniProt ID:	Q9Y2Q3
Clone ID:	24GB13635
Immunogen:	A synthesized peptide derived from human Glutathione S Transferase kappa 1
Dilution:	WB 1:500-1:2,500; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-GSTK1 antibody (Cat#64895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GSTK1 antibody (Cat#64895, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-GSTK1 antibody (Cat#64895). GSTK1 expression in wild-type (WT) and GSTK1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GSTK1 antibody (Cat#64895, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of GSTK1 expression in HepG2 cells using anti-GSTK1 antibody (Cat#64895, 1:1,000). Green, isotype control; red, GSTK1.