Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | BCL2 interacting protein 3 |
| Gene Symbol: | BNIP3 |
| Synonyms: | NIP3; HABON |
| Gene ID: | 664 |
| UniProt ID: | Q12983 |
| Clone ID: | 24GB13585 |
| Immunogen: | A synthesized peptide derived from human BNIP3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.
Images
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Western blotting analysis using anti-BNIP3 antibody (Cat#64885). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BNIP3 antibody (Cat#64885, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-BNIP3 antibody (Cat# 64885). BNIP3 expression in wild-type (WT) and BNIP3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BNIP3 antibody (Cat# 64885, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat# 201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat# 716). |
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Flow cytometric analysis of BNIP3 expression in HepG2 cells using anti-BNIP3 antibody (Cat#64885, 1:1,000). Green, isotype control; red, BNIP3. |
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