Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome.

Images

Western blotting analysis using anti-PSMA6 antibody (Cat#64853). Total cell lysates (15 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMA6 antibody (Cat#64853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-PSMA6 antibody (Cat#64853). PSMA6 expression in wild-type (WT) and PSMA6 shRNA knockdown (KD) HeLa cells with 15 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PSMA6 antibody (Cat#64853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

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