Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | CWC27 spliceosome associated cyclophilin |
| Gene Symbol: | CWC27 |
| Synonyms: | RPSKA; NY-CO-10; SDCCAG10; SDCCAG-10 |
| Gene ID: | 10283 |
| UniProt ID: | Q6UX04 |
| Clone ID: | 24GB8300 |
| Immunogen: | Recombinant protein of human CWC27 |
| Dilution: | WB 1:500-1:2,500; FC 1:1,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable peptidyl-prolyl cis-trans isomerase activity. Predicted to be involved in protein folding. Located in nucleoplasm. Part of U2-type precatalytic spliceosome and catalytic step 2 spliceosome.
Images
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Western blotting analysis using anti-CWC27 antibody (Cat#63874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CWC27 antibody (Cat#63874, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-CWC27 antibody (Cat#63874). CWC27 expression in wild-type (WT) and CWC27 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CWC27 antibody (Cat#63874, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of CWC27 expression in HeLa cells using anti-CWC27 antibody (Cat#63874, 1:1,000). Green, isotype control; red, CWC27. |
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Immunocytochemical staining of HepG2 cells with anti-CWC27 antibody (Cat#63874, 1:1,000). Nuclei were stained blue with DAPI; CWC27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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