Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	dolichyl-diphosphooligosaccharide–protein glycosyltransferase non-catalytic subunit
Gene Symbol:	DDOST
Synonyms:	OST; WBP1; AGER1; CDG1R; GATD6; OST48; OKSWcl45
Gene ID:	1650
UniProt ID:	P39656
Clone ID:	24GB8240
Immunogen:	Recombinant protein of human DDOST
Dilution:	WB 1:200-1:1,000; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Images

	Western blotting analysis using anti-DDOST antibody (Cat#63862). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDOST antibody (Cat#63862, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-DDOST antibody (Cat#63862). DDOST expression in wild-type (WT) and DDOST shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DDOST antibody (Cat#63862, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of DDOST expression in C2C12 cells using anti-DDOST antibody (Cat#63862, 1:1,000). Green, isotype control; red, DDOST.