Description

Reactivity:	Human
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	epsin 2
Gene Symbol:	EPN2
Synonyms:	EHB21
Gene ID:	22905
UniProt ID:	O95208
Clone ID:	24GB8190
Immunogen:	Recombinant protein of human EPN2
Dilution:	WB 1:500-1:2,500; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Images

	Western blotting analysis using anti-epsin 2 antibody (Cat#63852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epsin 2 antibody (Cat#63852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-epsin 2 antibody (Cat#63852). Epsin 2 expression in wild type (WT) and epsin 2 (EPN2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epsin 2 antibody (Cat#63852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Epsin 2 expression in HeLa cells using anti-Epsin 2 antibody (Cat#63852, 1:1,000). Green, isotype control; red, Epsin 2.