Background

The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

Images

	Western blotting analysis using anti-DNAJA2 antibody (Cat#63839). Total cell lysates (10 μg for C2C12 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNAJA2 antibody (Cat#63839, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-DNAJA2 antibody (Cat#63839). DNAJA2 expression in wild type (WT) and DNAJA2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNAJA2 antibody (Cat#63839, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of DNAJA2 expression in C2C12 cells using anti-DNAJA2 antibody (Cat#63839, 1:1,000). Green, isotype control; red, DNAJA2.

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