Description

Reactivity:	Human, Mouse, Rat
Applications:	WB
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	ariadne RBR E3 ubiquitin protein ligase 2
Gene Symbol:	ARIH2
Synonyms:	ARI2; TRIAD1
Gene ID:	10425
UniProt ID:	O95376
Clone ID:	24GB8000
Immunogen:	Recombinant protein of human ARIH2
Dilution:	WB 1:1,000-1:5,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet.

Images

Western blotting analysis using anti-ARIH2 antibody (Cat#63813). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARIH2 antibody (Cat#63813, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-ARIH2 antibody (Cat#63813). ARIH2 expression in wild type (WT) and ARIH2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARIH2 antibody (Cat#63813, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

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