Description

Reactivity:	Human
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	ATP synthase F1 subunit gamma
Gene Symbol:	ATP5F1C
Synonyms:	ATP5C; ATP5C1; ATP5CL1
Gene ID:	509
UniProt ID:	P36542
Clone ID:	24GB7990
Immunogen:	Recombinant protein of human ATP5C1
Dilution:	WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene ATP5C1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.

Images

	Western blotting analysis using anti-ATP synthase F1 subunit gamma antibody (Cat#63811). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP synthase F1 subunit gamma antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-ATP synthase F1 subunit gamma antibody (Cat#63811). ATP synthase F1 subunit gamma expression in wild type (WT) and ATP synthase F1 subunit gamma (ATP5F1C) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP synthase F1 subunit gamma antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of ATP synthase F1 subunit gamma expression in HepG2 cells using anti-ATP synthase F1 subunit gamma antibody (Cat#63811, 1:1,000). Green, isotype control; red, ATP synthase F1 subunit gamma.