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KD-Validated BDH2 Mouse mAb (20 μl)

$149.00

Cat. #: 63795

Description

Reactivity: Human
Applications: WB, FC
Host Species: Mouse
Isotype: IgG2a
Clonality: Monoclonal antibody
Gene Name: 3-hydroxybutyrate dehydrogenase 2
Gene Symbol: BDH2
Synonyms: DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
Gene ID: 56898
UniProt ID: Q9BUT1
Clone ID: 24GB7910
Immunogen: Recombinant protein of human BDH2
Dilution: WB 1:1,000-1:5,000; FC 1:1,000
Purification Method: Affinity purified
Concentration: Lot dependent
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.


Background

Enables 3-hydroxybutyrate dehydrogenase activity and NAD binding activity. Involved in epithelial cell differentiation and fatty acid beta-oxidation. Located in cytosol.


Images

BDH2 antibody Western blotting analysis using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
63795 WB KD 1 Western blotting analysis using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795). 3-hydroxybutyrate dehydrogenase 2 expression in wild type (WT) and 3-hydroxybutyrate dehydrogenase 2 (BDH2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
63795 FCM 1 Flow cytometric analysis of 3-hydroxybutyrate dehydrogenase 2 expression in HepG2 cells using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:1,000). Green, isotype control; red, 3-hydroxybutyrate dehydrogenase 2.


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