Background

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.

Images

	Western blotting analysis using anti-small ubiquitin like modifier 1 antibody (Cat#63746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-small ubiquitin like modifier 1 antibody (Cat#63746). Small ubiquitin like modifier 1 expression in wild-type (WT) and small ubiquitin like modifier 1 (SUMO1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Small ubiquitin like modifier 1 expression in HAP-1 cells using anti-Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000). Green, isotype control; red, Small ubiquitin like modifier 1.

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