Description

Reactivity:	Human
Applications:	WB
Host Species:	Mouse
Isotype:	IgG1 kappa
Clonality:	Monoclonal antibody
Gene Name:	RAB27A, member RAS oncogene family
Gene Symbol:	RAB27A
Synonyms:	GS2; RAM; RAB27; HsT18676
Gene ID:	5873
UniProt ID:	P51159
Clone ID:	24GB7165
Immunogen:	Recombinant protein of human RAB27
Dilution:	WB 1:1,000-1:5,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Images

Western blotting analysis using anti-RAB27A antibody (Cat#63744). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB27A antibody (Cat#63744, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-RAB27A antibody (Cat#63744). RAB27A expression in wild-type (WT) and RAB27A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAB27A antibody (Cat#63744, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

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