Description

Reactivity:	Human, Rat
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	transglutaminase 2
Gene Symbol:	TGM2
Synonyms:	TGC; tTG; G(h); hTG2; TG(C)
Gene ID:	7052
UniProt ID:	P21980
Clone ID:	24GB7130
Immunogen:	Recombinant protein of human Transglutaminase 2
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-transglutaminase 2 antibody (Cat#63732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-transglutaminase 2 antibody (Cat#63732, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-transglutaminase 2 antibody (Cat#63732). Transglutaminase 2 expression in wild-type (WT) and transglutaminase 2 (TGM2) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-transglutaminase 2 antibody (Cat#63732, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Transglutaminase 2 expression in HT-1080 cells using anti-Transglutaminase 2 antibody (Cat#63732, 1:2,000). Green, isotype control; red, Transglutaminase 2.