Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	clathrin heavy chain
Gene Symbol:	CLTC
Synonyms:	Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2
Gene ID:	1213
UniProt ID:	Q00610
Clone ID:	24GB6960
Immunogen:	A synthesized peptide derived from human Clathrin heavy chain
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Images

	Western blotting analysis using anti-clathrin heavy chain antibody (Cat#63697). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-clathrin heavy chain antibody (Cat#63697, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-clathrin heavy chain antibody (Cat#63697). Clathrin heavy chain expression in wild-type (WT) and clathrin heavy chain (CLTC) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-clathrin heavy chain antibody (Cat#63697, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Clathrin heavy chain expression in HepG2 cells using anti-Clathrin heavy chain antibody (Cat#63697, 1:2,000). Green, isotype control; red, Clathrin heavy chain.
	Immunocytochemical staining of HepG2 cells with anti-Clathrin heavy chain antibody (Cat#63697, 1:1,000). Nuclei were stained blue with DAPI; Clathrin heavy chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.