Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | annexin A1 |
| Gene Symbol: | ANXA1 |
| Synonyms: | ANX1; LPC1 |
| Gene ID: | 301 |
| UniProt ID: | P04083 |
| Clone ID: | 24GB6815 |
| Immunogen: | Recombinant protein of human Annexin A1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene ANXA1 encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.
Images
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Western blotting analysis using anti-annexin A1 antibody (Cat#63667). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#63667, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-annexin A1 antibody (Cat#63667). Annexin A1 expression in wild-type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A1 antibody (Cat#63667, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Annexin A1 expression in HepG2 cells using anti-Annexin A1 antibody (Cat#63667, 1:2,000). Green, isotype control; red, Annexin A1. |
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Immunocytochemical staining of HepG2 cells with anti-Annexin A1 antibody (Cat#63667, 1:1,000). Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. |
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