Description

Reactivity:	Human, Mouse, Rat
Applications:	WB
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	annexin A1
Gene Symbol:	ANXA1
Synonyms:	ANX1; LPC1
Gene ID:	301
UniProt ID:	P04083
Clone ID:	24GB6780
Immunogen:	Recombinant protein of human Annexin A1
Dilution:	WB 1:1,000-1:5,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The gene ANXA1 encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.

Images

Western blotting analysis using anti-annexin A1 antibody (Cat#63660). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#63660, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-annexin A1 antibody (Cat#63660). Annexin A1 expression in wild-type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A1 antibody (Cat#63660, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

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