Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Images

	Western blotting analysis using anti-heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635). Heterogeneous nuclear ribonucleoprotein F expression in wild type (WT) and heterogeneous nuclear ribonucleoprotein F (HNRNPF) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Heterogeneous nuclear ribonucleoprotein F expression in HepG2 cells using anti-Heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:2,000). Green, isotype control; red, Heterogeneous nuclear ribonucleoprotein F.
	Immunocytochemical staining of HepG2 cells with anti-Heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:1,000). Nuclei were stained blue with DAPI; Heterogeneous nuclear ribonucleoprotein F was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm.

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