Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	Jun proto-oncogene, AP-1 transcription factor subunit
Gene Symbol:	JUN
Synonyms:	AP1; p39; AP-1; cJUN; c-Jun
Gene ID:	3725
UniProt ID:	P05412
Clone ID:	24GB5760
Immunogen:	Recombinant protein of human JUN
Dilution:	WB 1:1,000-1:2,500; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Images

	Western blotting analysis using anti-JUN antibody (Cat#63465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-JUN antibody (Cat#63465, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
	Western blotting analysis using anti-JUN antibody (Cat#63465). JUN expression in wild type (WT) and JUN shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-JUN antibody (Cat#63465, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of JUN expression in HT-1080 cells using anti-JUN antibody (Cat#63465, 1:2,000). Green, isotype control; red, JUN.
	Immunocytochemical staining of HT-1080 cells with anti-JUN antibody (Cat#63465, 1:1,000). Nuclei were stained blue with DAPI; JUN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm.