Description

Reactivity:	Human, Rat
Applications:	WB, FC
Host Species:	Mouse
Isotype:	IgG1
Clonality:	Monoclonal antibody
Gene Name:	Cystathionine gamma-lyase
Gene Symbol:	CTH
Synonyms:	CGL; CSE
Gene ID:	1491
UniProt ID:	P32929
Clone ID:	24GB5620
Immunogen:	Recombinant protein of human CTH
Dilution:	WB 1:1,000-1:2,500; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The gene CTH encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.

Images

	Western blotting analysis using anti-cystathionine gamma-lyase antibody (Cat#63437). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cystathionine gamma-lyase antibody (Cat#63437, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
	Western blotting analysis using anti-cystathionine gamma-lyase antibody (Cat#63437). Cystathionine gamma-lyase expression in wild type (WT) and cystathionine gamma-lyase (CTH) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cystathionine gamma-lyase antibody (Cat#63437, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Cystathionine gamma-lyase expression in HepG2 cells using anti-Cystathionine gamma-lyase antibody (Cat#63437, 1:2,000). Green, isotype control; red, Cystathionine gamma-lyase.