Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	heat shock transcription factor 1
Gene Symbol:	HSF1
Synonyms:	HSTF1
Gene ID:	3297
UniProt ID:	Q00613
Clone ID:	24GB5445
Immunogen:	A synthesized peptide derived from human Phospho-HSF1 (Ser303/Ser307)
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.

Images

	Western blotting analysis using anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#63389). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#63389, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#63389). Phospho-HSF1 (Ser303/Ser307) expression in wild type (WT) and HSF1 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#63389, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). HSF1, heat shock transcription factor 1.
	Flow cytometric analysis of Phospho-HSF1 (Ser303/Ser307) expression in HepG2 cells using anti-Phospho-HSF1 (Ser303/Ser307) antibody (Cat#63389, 1:2,000). Green, isotype control; red,Phospho-HSF1 (Ser303/Ser307).
	Immunocytochemical staining of HepG2 cells with anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#63389, 1:1,000). Nuclei were stained blue with DAPI; Phospho-HSF1 (Ser303/Ser307) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.