Description

Reactivity:	Human
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	E2F transcription factor 4
Gene Symbol:	E2F4
Synonyms:	E2F-4
Gene ID:	1874
UniProt ID:	Q16254
Clone ID:	24GB5375
Immunogen:	A synthesized peptide derived from human E2F4
Dilution:	WB 1:500-1:2,500; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The protein E2F4 encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Images

	Western blotting analysis using anti-E2F transcription factor 4 antibody (Cat#63375). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-E2F transcription factor 4 antibody (Cat#63375, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-E2F transcription factor 4 antibody (Cat#63375). E2F transcription factor 4 expression in wild type (WT) and E2F transcription factor 4 (E2F4) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-E2F transcription factor 4 antibody (Cat#63375, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of E2F transcription factor 4 expression in HAP-1 cells using anti-E2F transcription factor 4 antibody (Cat#63375, 1:2,000). Green, isotype control; red, E2F transcription factor 4.