Background

This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis.

Images

	Western blotting analysis using anti-TIMM50 antibody (Cat#63361). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TIMM50 antibody (Cat#63361, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using Plus ECL Super Kit (636).
	Western blotting analysis using anti-TIMM50 antibody (Cat#63361). TIMM50 expression in wild-type (WT) and TIMM50 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-TIMM50 antibody (Cat#63361, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of TIMM50 expression in HepG2 cells using anti-TIMM50 antibody (Cat#63361, 1:2,000). Green, isotype control; red, TIMM50.
	Immunocytochemical staining of HepG2 cells with anti-TIMM50 antibody (Cat#63361, 1:1,000). Nuclei were stained blue with DAPI; TIMM50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

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