Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Images

	Western blotting analysis using anti-phospho-NDRG1 (Ser330) antibody (Cat#63306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-NDRG1 (Ser330) antibody (Cat#63306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phospho-NDRG1 (Ser330) antibody (Cat#63306). Phospho-NDRG1 (Ser330) expression in wild-type (WT) and NDRG1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-NDRG1 (Ser330) antibody (Cat#63306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). NDRG1, N-myc downstream regulated 1.
	Flow cytometric analysis of phospho-NDRG1 (Ser330) expression in HepG2 cells using anti-phospho-NDRG1 (Ser330) antibody (Cat#63306, 1:2,000). Green, isotype control; red, phospho-NDRG1 (Ser330).

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