Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | mitofusin 1 |
| Gene Symbol: | MFN1 |
| Synonyms: | hfzo1; hfzo2 |
| Gene ID: | 55669 |
| UniProt ID: | Q8IWA4 |
| Clone ID: | 24GB4970 |
| Immunogen: | A synthesized peptide derived from Rat Mitofusin 1(UniProt Entry: Q8R4Z9) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.
Images
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Western blotting analysis using anti-mitofusin 1 antibody (Cat#63303). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-mitofusin 1 antibody (Cat#63303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-mitofusin 1 antibody (Cat#63303). Mitofusin 1 expression in wild-type (WT) and mitofusin 1 (MFN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-mitofusin 1 antibody (Cat#63303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of Mitofusin 1 expression in C2C12 cells using anti-Mitofusin 1 antibody (Cat#63303, 1:2,000). Green, isotype control; red, Mitofusin 1. |
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